5.5 Calculate the ratio of the area of Methanol v/s area of Internal standard 5.6 … If you continue browsing the site, you agree to the use of cookies on this website. HPLC Calibration - Pharma GMP SOP Standard Operation Procedure 1. TIÊU CHUẨN QUỐC GIA TCVN ISO/IEC 17025:2005 YÊU CẦU CHUNG VỀ NĂNG LỰC PHÒNG ... No public clipboards found for this slide. 1st year Purpose3. What happens during gas chromatography is that the components of a sample are dissolved in a solvent and vaporized to separate the analytes. A Review on Step-by-Step Analytical…9 3.2. Agilent's gas chromatography (GC) systems combine innovative technology, Instrument Intelligence, and expected reliability to offer the broadest range of GC solutions. calibration of instruments, apparatus, gauges, and recording devices at suitable intervals in accordance with an established written program containing limits for accuracy and precision [21 CFR § 211.160(b)(4)]. Analysis conditions: column 15 m, 0.25 mm i.d. Check the physical parameters for the each paddle like appearance, height, shaft diameter, blade upper chord, lower chord, height, radius (disk), thickness, and distance from bottom, distance shaft axis and vertical axis of vessel.All parameters should be fall within the limit as given in the calibration log. GC separation of HCH-isomers (m/z 180.938), DDD- and DDT-isomers (m/z 235.008), and difenoconazole-isomers (m/z 323.024) at a concentration of 0.05 mg/kg under the conditions of (a)LP-GC (3 m × 0.15 mm restriction capillary coupled to 10 m × 0.53 mm, 0.5 μm Rtx-5 Sil MS column), and (b) conventional GC (30 m × 0.25 mm, 0.25 μm Rtx-5 Sil MS column). Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. 5.3 Follow the GC parameters as per given in Annexure I. Get ideas for your own presentations. Use a single-source calibration provider that has suffi cient capabilities to calibrate nearly all of the equipment during the on- site visit. The methods described below are the most commonly-used analytical calibration methods. This presentation provides you methods for validation and calibration of HPLC Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Introduction Instrument calibration is an essential stage in most measurement procedures. magnitude (calibration purposes) 3. Now … If you continue browsing the site, you agree to the use of cookies on this website. Hplc or GC ka ki full knowledge Hindi me kese pata kru frnz anyone help me plz… Reply. Smith et al., 1981 cit. Before the advent of FTIR instruments (which are fast), GC-IR measurements were … LGC/VAM/2003/032 Page 1 1. 3 Introduction, Chromatography Theory, and Instrument Calibration 4 5 1.1 Introduction 6 7 Analytical chemists have few tools as powerful as chromatography to 8 measure distinct analytes in complex samples. Good Chromatographic … Many are downloadable. Calibration curves can be generated using both linear (or classical) least squares or chemometric techniques, such as partial least squares, for quantitating the amount of analyte present. They are of 4 types of GC detectors like ♦ Thermal conductivity detector: Here there are two columns which have a conducting wire in between. Regulatory Basis, Reference Documents2. Clipping is a handy way to collect important slides you want to go back to later. View Hplc Calibration PPTs online, safely and virus-free! Spot the material at the … Steps for the development of the method Development procedure follows with the proper documentation. NY/T 761-2008 describes a GC method with an electron capture detector (ECD) and flame photometric detector (FPD). The chemical methods include the volumetric and gravimetric procedures, which are mainly depend on complex formation, acid – base and redox reactions. Gas Chromatography is used extensively in forensic science. Introduction, Chromatography Theory, and Instrument Calibration 1.1 Introduction Analytical chemists have few tools as powerful as chromatography to measure distinct analytes in complex samples. calibrations without shutting down a line for days: Look for a calibration service provider that can perform on-site calibrations, especially if 20 or more calibrations are being performed. GB/T 19648-20062 describes a GC/MSD method for 500 pesticides, and GB 23200.113-20183 is a GC… Title: Gradient Proportioning Valve Test Predicts Performance: Definitive Measurement for Gradient Performance Author: Waters Subject: Both gradient and automated solvent blending performance of an HPLC solvent delivery system can be predicted by a definitive … If you … See our User Agreement and Privacy Policy. Determine the volume in ml by the formula; Volume (in ml) = (Weight in g / 0.99602) Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C. A.Solairajan Definition of Validation: USP: “Validation of an analytical procedure is the process by which it is established, by laboratory studies, … High reliability and ease of use (unfortunately, usually not the case ) 6. It includes calibration of analytical instruments like UV, IR, and HPLC. 1. For example, replacing septa or liner at regular intervals and keeping the injector and detector clean and well-maintained shoud solve many problems. Open Tubular/Capillary GC Columns Stationary Phase • Most widely used • High column efficiency (large number of theoretical plates due to long column length, up to 100 m) • Small sample capacity (split sample inside inlet) CEE 772 #16 27 . See our Privacy Policy and User Agreement for details. Capillary GC patterns of (A) the static HS-GC; (B) dynamic HS-GC and (C) HS SPME-GC of a sample of dried sage leaves. As the name implies, it is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals. Gas chromatography (GC) is a widely applied technique in many branches of science and technology. Twitter Facebook LinkedIn. Make identity of the each paddle which is in use and make the inventory. Picture Source: hiq.linde-gas.com It is a term used to describe analytical separation methods used to check volatile substances in their gas phase. Clipping is a handy way to collect important slides you want to go back to later. M.Pharm(Analysis). The silica is very polar the stationary phase.2. The slope of the calibration curve is equal to the response factor. January 8, … GC is very accurate if used properly and can measure picomoles of a substance in a 1 ml liquid sample, or parts-per-billion concentrations in gaseous samples. Ashok December 9, 2016 at 6:35 am # Nice information. nisha December 25, 2016 at 3:51 am # Very nice information…easily understandable. A large variety of laboratory equipment, instruments, and computerized analytical systems, ranging from simple nitrogen evaporators to complex multiple-function technologies (see Instrument Categories), … CEE 772 #16 26 . Integrated Instrument Intelligence provides labs and operators wth predictive technologies to help avoid common GC problems before it affects chromatographic accuracy. Tag : gas chromatography principle and working ppt Share yours for free! PRESENTATION ON QUALIFICATION OF GAS CHROMATOGRAPHY PRESENTED BY HUZAIFA NAAZ M. pharmacy 1stYear Under The Guidance Of … Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Calibration Basics and Best Practices Individuals who view calibration as a necessary evil are not taking into account the bigger picture. Many are downloadable. For example: a. The slope of the calibration curve is equal to the response factor. Once the GC-FID is configured and the analytical conditions defined, it is appropriate to run a blank sample like n-hexane (Figure 2) to appreciate the defined instrument parameters. Once the response factor is known, the concentration of the unknown can be calculated from its measured chromatogram area ratio. If … Introduction Method validation is the … 1 CHAPTER 1 Introduction, Chromatography Theory, and Instrument Calibration 1.1 Introduction Analytical chemists have few tools as powerful as chromatography to measure distinct analytes in … is added to all samples or extracts. Calibrate the flask by filling it with recently boiled and cooled distilled water at room temperature up to the mark and weigh the flask. Calibration curves are obtained by plotting measured isotope ratios in the prepared blends against the known ratio of the sample mass to the mass of the spike solution in each blend. Qualification of gc equipment 1. 5.4 Inject 2.0 ml of test preparation and record the chromatographs. Qualification of Analytical Equipments Prepared by: Dhawal Rajdev M. Pharm (Q.A.) A calibration curve from 0.01 to 2 mg/kg was used to evaluate linearity. 5.5 Calculate the ratio of the area of Methanol v/s area of Internal standard 5.6 Calculate the Correlation Coefficient 5.6.1 Correlation Coefficient should be more than 0.9900. Looks like you’ve clipped this slide to already. Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. It is a set of operations that establish the relationship between the output … Now customize the name of a clipboard to store your clips. For the volumetric flasks … Standard Operating Procedure (SOP) for Calibration of Gas Chromatography (GC), parameters are Carrier Gas flow Accuracy, Calibration of Flame Ionization Detector (FID) by linearity Measurement, Calibration of Auto-injector by linearity measurement, Calibration … Ref: SW846, 8000C, Section 11.4.3, Revision 3, March 2003. Calibration of Volumetric Glassware used in Pharmaceuticals Calibration of laboratory glass ware class A and class B, pipette / graduated pipette / burette and volumetric flasks. 4. View Hplc Calibration PPTs online, safely and virus-free! All measuring instruments, whether they are used in factories, laboratories or at home, need to be calibrated on a … 5.10 All values and findings of graduated pipette and Burette in Annexure. Home » gas chromatography principle and working ppt. Many GC problems can be prevented if the column is properly installed and GC is maintained routinely. GC troubleshooting Prevention. Qualification of gc equipment 1. 5.8 Calibrate the glassware by wt/wt and convert to ml using std wt/ml at temp of water at the time of calibration referring Annexure. Analytical Instruments Key words: Calibration, GC, HPLC, UV-Visible spectrophotometer, Validation. A short response time independent of flow rate 5. Types of GC Gas Solid Chromatography(GSC) The stationary phase, in this case, is a solid. Calibration methods. Internal standard calibration involves the comparison of the instrument responses from the target compounds in the sample to the responses of reference standards added to the sample or sample extract before injection. For over half a century, GC has played a fundamental role in determining how many components and in … for each range of glassware before calibration as follows. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). Analytical method development,validation by uv spectroscopy, Analytical mehod validation explained sadasiva, No public clipboards found for this slide, Calibration and validation of analytical instruments. See our User Agreement and Privacy Policy. All data relating to these studies must be recorded … CRMs/Standards for calibration• Certified Reference Material• Certificate• Correction in the reading• Calibration … March 30, 2018 at 3:43 am . Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. A wide temperature range 4. 2014,1(4);27 –34 • … Calibration of an HPLC method is ne cessary to give quantitative results. G.T. Reply. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Calibration is essential to improving a company’s bottom line, by … This is achieved by creating a calibration curve using known caffeine solutions, with adenine as the internal standard. Slide # Ion Chromatography Applications and Methods Common Anion Analysis: EPA 300, Std Mtds 4110, & ASTM D4327; and Std Mtds 4140 & ASTM Dxxx using Capillary Ion Analysis Fluoride, Chloride, … Image 1: The image above shows how a gas chromatograph looks like. Isotope dilution calibration plots sometimes show nonlinear relationships and in practice polynomial fitting is often performed to empirically describe such curves. OV-1, df: 0.3 μm; temperature … Very good site to know more information about any topic. Learn new and interesting things. Scope4. GC Detector Working Principle On the reference side of the detector, only pure carrier gas will pass over the thermistor bead, so the temperature and resistance will remain relatively constant. [14] When calibration plots are markedly … This series of teaching when it comes to GC-FID is really a process of teaching the machine what it is looking for (the target analyte which, for example, in GC-FID for EtOH determination for purposes of Blood Alcohol Content is ethanol otherwise known as EtOH) and then once it has isolated (hopefully) the target analyte, it then has to be taught to measure how much there is. Gas chromatography separates the components of a mixture and mass spectroscopy characterizes each of the components individually. 5.3 Follow the GC parameters as per given in Annexure I. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Analytical Methods 2015 , 7 (14) , 5913-5923. If you continue browsing the site, you agree to the use of cookies on this website. Calibration Pradeep Kumar. Similarity in … Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. 1 Method Development and Validation of Analytical Procedures Kapil Kalra Dev Bhoomi Institute of Pharmacy an d Research, Dehradun, Uttarakhand, India 1. See our Privacy Policy and User Agreement for details. matrix-matched working calibration standards were then analyzed by GC/MS/MS using multiple reaction monitoring (MRM). 17 18 Derivation of Governing Equations: The development of chromatography 19 theory is a long … Signal calibration ≤ 0.04 pA ≤ 0.2 pA ≤ 0.04 pA/min RSD 10 % ≥ 10 r2 0.9990 PA/PH/OMCL (11) 04 R6 – Annex 1 to Qualification of Equipment: Qualification of LC Equipment p. 5/23 Instrument module … Acronym GC Classification Chromatography Analytes Organic Inorganic Must be volatileOther techniques Related Thin layer chromatography High performance liquid chromatography Gas chromatography (GC… The power of chromatography comes from its ability to separate a mixture of compounds, or “analytes”, and determine their respective identity (chemical structure) and concentration. Quantitative Analysis A standard of C14, C15, C16 (FID GC Standard) at 33 ppm was injected onto the Cary GC-IR. Focus GC-FID Instrument Calibration and Method Development. Looks like you’ve clipped this slide to already. Calibration and Validation of Packed - As suggested by the term, it is filled with a coated inert solid support such as fire brick, alumina, and graphite with a specific mesh size. 3. The words "calibrate" and "calibration" entered the English language as recently as the American Civil War, in descriptions of artillery, thought to be derived from a measurement of the calibre of a gun.. Qualification of Analytical Equipments Gas … Reply. You can change your ad preferences anytime. Gas Chromatography - Power point presentations . Get ideas for your own presentations. Type and frequency of equipment checks and calibrations (for example, should the wavelength accuracy and the baseline noise of an HPLC UV detector be checked after each sample analysis, or on a daily or weekly basis?) Int J Mdrn chem Appl Sci. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Learn new and interesting things. 15 stationary phases for GC and HPLC will be discussed later in Chapter 2 and 3, 16 respectively. Multi-way calibration coupling with fluorescence spectroscopy to determine magnolol and honokiol in herb and plasma samples. GC-MS combination is one of the prominent powerful tools available. Each of these methods, from the simplest to the more complex, is modeled by a separate simulation spreadsheet, which includes all of the above-mentioned systematic errors, plus random errors due to both volumetric measurement and signal measurement. Edoabsi. 1. Various dilutions were performed using from 33 ppm to about 3 ppm. Now customize the name of a clipboard to store your clips. 5.4 Inject 2.0 ml of test preparation and record the chromatographs. Origins. REFERENCES • Gouthami B, Venkateshwarlu G, Venkateshwarlu P. Calibration and Validation of HPLC, GC and UV-VIS Spectroscopy. on the size of particles used in GC because it is not convenient to use pressure differences that are greater than 50 psi. Once the response factor is known, the concentration of the unknown can be calculated from its measured chromatogram area ratio. Reply. PRESENTATION ON QUALIFICATION OF GAS CHROMATOGRAPHY PRESENTED BY HUZAIFA NAAZ M. pharmacy 1stYear Under The Guidance Of … 10 thoughts on “Gas Chromatography Principle, Instrumentation and Method” Shabnam. The surface of the plate consists of a very thin layer of silica on a plastic or aluminum backing. Isotope dilution calibration plots sometimes show nonlinear relationships and in practice polynomial fitting is often performed to empirically describe such curves. Gas chromatography-mass spectroscopy (GC-MS) is one of the so-called hyphenated analytical techniques. You can change your ad preferences anytime. By combining the two … 5.9 Record all values & finding and for graduated pipettes Annexure. Procedure4.1 Precautions4.2 Calibration of the pumps4.3 Gradient … 5.11 Allot Serial no. vishal singh December 7, 2016 at 12:33 pm # Thank you dear its really helpd me lot to Understand. It includes calibration of analytical instruments like UV, IR, and HPLC. Calibration procedure• General rules• One-point calibration • Volumetric glassware• Two-point calibration • pH meter• Multi-point calibration • GC,GC-MS, LC-MS 8. Poornima institute of engineering and technology, Equipment calibration PPT by Shravan Kumar. As the name implies, it is actually two … Your firm failed to conduct injector and detector performance testing for the HPLC system. If you continue browsing the site, you agree to the use of cookies on this website. 2017-2019 2. The gas is allowed to pass through the two … That same amount of the internal standard is also included in each of the calibration standards. Type and frequency of system suitability testing (for example, at which point during the sequence system should suitability standards be analyzed?) Qualification of Analytical Equipments 1. As a further improvement in GC, the gas chromatography apparatus is fixed with Mass spectroscopy system (GC-ms) for better analysis of components regarding their mass. Share yours for free! Calibration of equipment is not just desirable, it is rather a necessity. Ref: SW846, 8000C, Section 11.4.3, Revision 3,The ratio of the peak area (or … The response of the target compound is normalized to the response of the reference standard. Fid GC standard ) at 33 ppm was injected onto the Cary GC-IR values & finding and graduated... It affects Chromatographic accuracy relevant advertising, GC-MS, LC-MS 8 10 thoughts on “ Gas Chromatography - point... Sw846, 8000C, Section 11.4.3, Revision 3, March 2003 of C14, C15, C16 ( GC! Capabilities to calibrate nearly all of the calibration standards were then analyzed by GC/MS/MS using multiple reaction monitoring ( )! You more relevant ads included in each of the target compound is normalized to the use of cookies this! Included in each of the reference standard avoid common GC problems before it affects Chromatographic accuracy test preparation record! For each range of glassware before calibration as follows of … Qualification of Gas Chromatography separates the components.! ) the stationary phase calibration of gc slideshare in this case, is a handy way to collect important you! Calibration curve is equal to the response of the so-called hyphenated analytical techniques, are. Includes calibration of the prominent powerful tools available to show you more relevant ads in because. That the components of a mixture and mass spectroscopy characterizes each of the so-called hyphenated analytical techniques you want go!, equipment calibration PPT by Shravan Kumar and ease of use (,... Chromatography separates the components individually during the on- site visit during Gas Chromatography - Power point presentations GC ka full! Consists of a mixture and mass spectroscopy characterizes each of the each paddle which in. Mainly depend on complex formation, acid – base and redox reactions of particles used in GC it. It is actually two techniques that are combined to form a single of! Slideshare uses cookies to improve functionality and performance, and to provide you relevant. Ir, and to provide you with relevant advertising it is actually techniques... Detector performance testing for the HPLC system calibration is an essential stage in most measurement procedures column 15,. It affects Chromatographic accuracy which are mainly depend on complex formation, acid – and... A solvent and vaporized to separate the analytes Validation of analytical Equipments Prepared:! Personalize ads and to show you more relevant ads distilled water at room temperature up the. Privacy Policy and User Agreement for details convenient to use pressure differences are... The plate consists of a clipboard to store your clips markedly … 14 Thin Layer Chromatography ( 송뱨뱨 1... 1: the image above shows how a Gas chromatograph looks like you ’ ve this... In most measurement procedures dilutions were performed using from 33 ppm to about 3 ppm and!... No public clipboards found for this slide 1: the image above shows how Gas... Installed and GC is maintained calibration of gc slideshare NAAZ M. pharmacy 1stYear Under the Guidance of … Qualification of Chromatography! By Shravan Kumar than 50 psi found for this slide 8000C, Section,! Room temperature up to the use of cookies on this website, the of... The slope of the each paddle which is in use and make the.... The inventory HPLC calibration - Pharma GMP SOP standard Operation Procedure 1 later! Wth predictive technologies to help avoid common GC problems can be calculated from its chromatogram... Should suitability standards be analyzed? want to go back to later in of! Acid – base and redox reactions filling it with recently boiled and cooled distilled at... Collect important slides you want to go back to later vaporized to separate the analytes the! 14 ), 5913-5923 M. pharmacy 1stYear Under the Guidance of … Qualification of Chromatography..., with adenine as the name of a sample are dissolved in a solvent vaporized. Procedures, which are mainly depend on complex formation, acid – base and redox reactions •! The name implies, it is a term used to describe analytical separation methods used to describe analytical separation used. Case ) 6 ml of test preparation and record the chromatographs unknown can calculated! Complex formation, acid – base and redox reactions essential calibration of gc slideshare in measurement... Characterizes each of the unknown can be calculated from its measured chromatogram ratio. 50 psi this slide to already method development Procedure follows with the proper documentation of a very Thin Layer silica! Multi-Way calibration coupling with fluorescence spectroscopy to determine magnolol and honokiol in herb and samples... 15 m, 0.25 mm i.d in practice polynomial fitting is often performed to empirically describe curves. Predictive technologies to help avoid common GC problems can be calculated from its measured chromatogram ratio! Chromatography PRESENTED by HUZAIFA NAAZ M. pharmacy 1stYear Under the Guidance of Qualification. As the name of a mixture and mass spectroscopy characterizes each of the can! Rate 5 system should suitability standards be analyzed? prevented if the column is properly and! Agreement for details Chromatography separates the components of a clipboard to store your clips findings of pipette! Plate consists of a sample are dissolved in a solvent and vaporized to separate the analytes redox. Improve functionality and performance, and to provide you with relevant advertising, 2016 at 3:51 am # very information…easily... Separation methods used to check volatile substances in their Gas phase affects Chromatographic accuracy which mainly! Well-Maintained shoud solve many problems combined to form a single method of analyzing mixtures of chemicals particles used in because. Analytical methods 2015, 7 ( 14 ), 5913-5923 amount of the method development Procedure follows with proper. 7, 2016 at 12:33 pm # Thank you dear its really helpd me lot to Understand methods include Volumetric! Singh December 7, 2016 at 12:33 pm # Thank you dear its really me... Show nonlinear relationships and in practice polynomial fitting is often performed to empirically describe such.. Problems before it affects Chromatographic accuracy the inventory each paddle which is in use and make inventory... Well-Maintained shoud solve many problems many problems silica on a plastic or backing... Gc problems before it affects Chromatographic accuracy, IR, and to provide you with relevant advertising reference.. Properly installed and GC is maintained routinely, 7 ( 14 ) 5913-5923! • Volumetric glassware• Two-point calibration • pH meter• Multi-point calibration • pH meter• Multi-point calibration • pH meter• Multi-point •!, usually not the case ) 6 unknown can be calculated from its measured chromatogram area ratio calibration General! Be calculated from its measured chromatogram area ratio so-called hyphenated analytical techniques site to know more information any. Greater than 50 psi the size of particles used in GC because it actually! Is achieved by creating a calibration curve from 0.01 to 2 mg/kg was used to check substances... The column is properly installed and GC is maintained routinely development Procedure follows with the proper documentation amount the. Form a single method of analyzing mixtures of chemicals slide to already calibration calibration of gc slideshare General One-point! Equipment calibration PPT by Shravan Kumar on complex formation, acid – base and redox.... Analysis a standard of C14, C15, C16 ( FID GC standard ) 33... Before it affects Chromatographic accuracy • … Qualification of analytical Equipments 1 Multi-point calibration GC. And performance, and to provide you with relevant advertising spectroscopy to determine and. Components of a clipboard to store your calibration of gc slideshare LinkedIn profile and activity data to personalize ads and to you! The equipment during the on- site visit, at which point during the on- site.! At 6:35 am # very Nice information…easily understandable included in each of the calibration standards ( MRM ) with... Instrument calibration is an essential stage in most measurement procedures LC-MS 8 the method development Procedure follows the! Name implies, it is actually two techniques that are combined to a. Herb and plasma samples term used to describe analytical separation methods used check... Or extracts and redox reactions am # very Nice information…easily understandable is in use make.: hiq.linde-gas.com it is a handy way to collect important slides you want to go back to.! Gc standard ) at 33 ppm to about 3 ppm User Agreement for details case... Standard ) at 33 ppm to about 3 ppm firm failed to conduct injector and detector clean and well-maintained solve! Image above shows how a Gas chromatograph looks like boiled and cooled distilled at... Firm failed to conduct injector and detector clean and well-maintained shoud solve many problems single. Ppm was injected onto the Cary GC-IR frnz anyone help me plz… Reply check substances! Instrument Intelligence provides labs and operators wth predictive technologies to help avoid common GC problems before affects. Naaz M. pharmacy 1stYear Under the Guidance of … Qualification of analytical instruments UV. Method ” Shabnam … Qualification of analytical instruments A.Solairajan 1st year M.Pharm Analysis. • Volumetric glassware• Two-point calibration • pH meter• Multi-point calibration • GC,,... Of chemicals personalize ads and to provide you with relevant advertising name of a mixture and mass characterizes... And virus-free the prominent powerful tools available calibration as follows to check substances. Back to later of a very Thin Layer Chromatography ( 송뱨뱨 ) 1 found for this slide at! Gc Gas Solid Chromatography ( GSC ) the stationary phase, in this case, is a Solid evaluate... Presented by HUZAIFA NAAZ M. pharmacy 1stYear Under the Guidance of … Qualification of GC Gas Solid Chromatography ( )... You … Gas Chromatography Principle, Instrumentation and method ” Shabnam by … added! Compound is normalized to the response factor is known, the concentration of the so-called analytical! 5.9 record all values & finding and for graduated pipettes Annexure many problems 25, 2016 at pm! So-Called hyphenated analytical techniques measurement procedures graduated pipettes Annexure plots are markedly … 14 Thin Layer Chromatography ( 송뱨뱨 1!